A negative pressure wound dressing is provided. The negative pressure wound dressing sequentially includes a backing layer, a water blocking layer, an absorbing layer and a wound contacting layer. Wherein, the backing layer comprises a connecting hole for fluidly connecting a negative pressure source. The wound contacting layer comprises a surface layer for contacting wound and a plurality of chambers, the opening of chambers are disposed in the surface layer for contacting wound and a pharmaceutical is contained in the chambers. The pharmaceutical can be prevented from being attaching to absorbing layer and be released toward the wound to improve healing the wound by using the negative pressure wound dressing of the present invention.一種負壓傷口敷料,其結構依序包含有一背材、一阻水層、一吸收層以及一傷口接觸層。其中,背材具有一連接孔,用以流體地連接一負壓源。傷口接觸層具有一傷口接觸之表層且包含複數個腔室,腔室之開口位於傷口接觸層之表層且腔室容置有藥劑。利用本創作之傷口敷料可防止藥劑因負壓而被吸引至吸收層,使藥劑可朝向傷口處釋放,進而促進傷口癒合。100‧‧‧背材110‧‧‧連接孔200‧‧‧阻水層300‧‧‧吸收層400‧‧‧傷口接觸層410‧‧‧表層420‧‧‧腔室421‧‧‧開口430‧‧‧藥劑