Method for in vivo labeling of defined cell types in a tissue, useful for detecting specific cells and to establish expression profiles, by providing cell-specific expression of fluorescent protein
Method for in vivo labeling of a defined cell type within a tissue by cell-specific expression of at least one nucleic acid (I) that expresses a subcellularly localized autofluorescent protein (II) in a transgenic non-human mammal. - Method for in vivo labeling of a defined cell type within a tissue by cell-specific expression of at least one nucleic acid (I) that expresses a subcellularly localized autofluorescent protein (II) in a transgenic non-human mammal. (I) comprises (a) a cell-type specific promoter (P); (b) a sequence (III) that encodes (II), functionally linked to (P); and (c) a sequence (IV) encoding an amino acid sequence (V) that directs subcellular localization of (II), attached to (III) at either end or internally. - Independent claims are also included for the following: - (1) nucleic acid (IIIa) that comprises (III), (IV) and a promoter, particularly (P); - (2) expression vector that contains (IIIa); - (3) method for preparing a transgenic, non-human mammal carrying a transgene for (II), by incorporation of (IIIa) into a chromosome; - (4) transgenic, non-human mammal, either produced by method (3), or carrying a transgene that encodes a (II) that is localized in or on cell membranes or organelles and/or on cell filaments; and - (5) method for preparing an expression profile for a particular cell type within a tissue.
