The present invention relates to a non-peptidyl polymer-insulin multimer comprising two or more of a non-peptidyl polymer-insulin conjugate that is prepared by linking a non-peptidyl polymer and insulin via a covalent bond, in which the conjugates are complexed with cobalt ion to form a multimer, a method and kit for the preparation of the multimer, a pharmaceutical composition for the prevention or treatment of diabetes comprising the multimer as an active ingredient, and a method for preventing or treating diabetes by administering the composition to a subject having diabetes or suspected of having diabetes. After subcutaneous injection, the multimer of the present invention has a large volume before dissociation into monomers by natural dilution, and thus its rapid absorption into the bloodstream does not occur. Accordingly, a large amount of the drug can be given at once. In addition, since it has a property of slow dissociation into monomers, it is useful in the development of long-acting insulin formulations.本發明係有關非肽基聚合物-胰島素集合體,其包含兩個或更多個藉由共價鍵連接非肽基聚合物與胰島素所製成之非肽基聚合物-胰島素接合物,其中該接合物係與鈷離子錯合形成集合體;亦有關製備該集合體之方法與套組、包含該集合體作為活性成份之供預防或治療糖尿病之醫藥組成物以及藉由對罹患或疑似罹患糖尿病之個體投予該組成物以預防或治療糖尿病之方法。於皮下注射後,本發明之集合體在自然稀釋解離成單體前,具有有大體積,因而不會被快速吸收至血流中。因此,可一次投予大量藥物。此外,由於其具有緩慢解離成單體之性質,因此其適用於發展長效胰島素製劑。該代表圖無元件符號及其所代表之意義。
