GRILLBERGER LEOPOLD,REITER MANFRED,FLEISCHANDERL DANIEL,BRAMBERGER GREGOR
申请号：
NZ60540311
公开号：
NZ605403A
申请日:
2011.07.08
申请国别(地区):
NZ
年份:
2014
代理人:
摘要：
Discloses a method of producing a recombinant ADAMTS13 (rA13) composition, the method comprising (a) providing a basal cell culture media； (b) supplementing the basal cell culture media with copper to provide a supplemented basal cell culture media with a final copper concentration of from 1.0 ug/L to 20 ug/L； (c) providing one or more cells comprising a nucleic acid encoding a rA13 protein； (d) culturing the one or more cells in the supplemented basal cell culture media such that the rA13 protein is expressed and excreted from the cells into a cell culture supernatant, wherein the NH4 + content of the cell culture supernatant is maintained at a concentration below 10 mM； and (e) recovering at least a portion of the cell culture supernatant, wherein at least 1500 Units FRETS-VWF73 activity per litre of the supplemented basal cell culture media per day is present in the cell culture supernatant recovered.
