DNA MARKER OF ADIPOCYTE-FATTY ACID BINDING PROTEIN GENE RELATED THE INTRAMUSCULAR FAT CONTENT IN BEEF CATTLE
- 专利权人:
- REPUBLIC OF KOREA(MANAGEMENT : RURAL DEVELOPMENT ADMINISTRATION)
- 发明人:
- PARK, EUNG WOO,YUN, DU HAK,LEE, SEUNG HWAN,CHO, YONG MIN,OH, SUNG JONG
- 申请号:
- KR20060065802
- 公开号:
- KR20080006719(A)
- 申请日:
- 2006.07.13
- 申请国别(地区):
- 韩国
- 年份:
- 2008
- 代理人:
- 摘要:
A DNA marker is provided to determine an individual genetic trait of Korean native cattle by using genotypes of three independent loci of a FABP4(adipocyte-fatty acid binding protein) gene which has the significantly effect for carcass weight and marbling of the Korean native cattle, thereby being utilized for an early selection of a fatting beef and an accurate estimate of breeding values. A method for selecting a DNA marker of determining genetic traits comprises the steps of: (a) identifying a genotype of a linkage group detected from a Korean native cattle a fatty acid binding protein(FABP4) gene of SEQ ID : NO. 7 using the decoding of 17 SNP loci depicted at a Fig. 1; (b) after pre-denaturing the Korean native cattle FABP4 gene of SEQ ID : NO. 7 as a template using a PCR primer of SEQ ID : NOs. 1 to 6 at a temperature of 94 deg.C for 5 minutes, performing 35 times of a cycle where one minute annealing at a temperature of 58 deg.C and two-minute expanding at a temperature of 72 deg.C are done and then
- 来源网站:
- 中国工程科技知识中心