BERTOLOTTI, ANNE,DAS, INDRAJIT,KRZYZOSIAK, AGNIESZKA,ROUSSEAU, ADRIEN,SCHNEIDER, KIM,SIGURDARDOTTIR, ANNA GUDNY
申请号：
CA2982033
公开号：
CA2982033A1
申请日:
2016.04.08
申请国别(地区):
CA
年份:
2016
代理人:
摘要：
The present invention discloses a method to discover selective inhibitors of phosphatases. Thus the invention provides a method for screening a test compound to determine whether the compound binds a holophosphatase selectively or non-selectively comprising: i) providing a first holophosphatase wherein said holophosphatase is captured/immobilised； ii) testing a test compound for its ability to bind to the first holophosphatase； iii) providing a second holophosphatase wherein said second holophosphatase is captured/immobilised； iv) testing the same test compound for its ability to bind to the second holophosphatase； v) comparing the binding of the test compound to said first holophosphatase with the binding to said second phosphatase wherein a compound that binds a holophosphatase selectively will bind to said first holophosphatase but not said second holophosphatase； or will bind to said second holophosphatase but not said first； or wherein a compound that binds a holophosphatase non-selectively will bind to both said first holophosphatase and said second holophosphatase.
