The present invention discloses a method of generating an animal fatty liver model. The steps of the method comprise: feeding an animal on a high caloric diet, and injecting a mitochondrial inhibitor into the animal to inhibit the mitochondrial activity of the animal, then resulting in liver fat accumulation and inflammatory reaction of the animal.本發明係揭露一種產生動物脂肪肝模式之方法,其步驟包括:以高熱量飼料餵食動物,再將粒線體之抑制劑注射至動物體內,使抑制劑抑制動物之粒線體活性,進而造成動物之肝臟脂肪堆積與產生發炎反應。S11~S12‧‧‧步驟