PROBLEM TO BE SOLVED: To provide a manufacture method of an acute severe hepatitis model nonhuman animal capable of advancing an abrupt damage of a human hepatocyte, the acute severe hepatitis model nonhuman animal, a screening method for a fulminant hepatitis curative medicine, and the fulminant hepatitis curative medicine.SOLUTION: A manufacture method of an acute severe hepatitis model nonhuman animal comprises: a first administration step of administrating an immunity administration to a super-immune deficient non-human animal, in which a human hepatocyte is applied to a liver and a second administration step of administrating a human being peripheral blood mononuclear ball to the super-immune deficient non-human animal, to which the immunity administration was administrated.SELECTED DRAWING: Figure 1COPYRIGHT: (C)2016,JPO&INPIT【課題】ヒト肝細胞の急激な傷害を進行させることができる急性重症肝炎モデル非ヒト動物の作製方法、急性重症肝炎モデル非ヒト動物、劇症肝炎治療薬のスクリーニング方法および劇症肝炎治療薬を提供する。【解決手段】急性重症肝炎モデル非ヒト動物の作製方法は、ヒト肝細胞を肝臓に生着させた超免疫不全非ヒト動物に、免疫刺激物質を投与する第1の投与工程と、前記免疫刺激物質を投与した前記超免疫不全非ヒト動物に、ヒト末梢血単核球を投与する第2の投与工程と、を含む。【選択図】図1
