The present invention provides a micro biosensor (10) for reducing a measurement interference when measuring a target analyte in the biofluid, including: a substrate (110); a first working electrode (120) configured on the surface (111), and including a first sensing section (121); a second working electrode (130) configured on the surface (111), and including a second sensing section (131) which is configured adjacent to at least one side of the first sensing section (121); and a chemical reagent covered on at least a portion of the first sensing section (121) for reacting with the target analyte to produce a resultant. When the first working electrode (120) is driven by a first working voltage, the first sensing section (121) measures a physiological signal with respect to the target analyte. When the second working electrode (130) is driven by a second working voltage, the second conductive material (2C) can directly consume the interferant so as to continuously reduce the measurement inference of the physiological signal.
