Chinping Chng,William Casey Hallows,Nicholas J. Agard,Oscar Alvizo,Nikki Dellas,Gjalt W. Huisman,John Joseph Nicols
申请号：
US15894446
公开号：
US20180230448A1
申请日:
2018.02.12
申请国别(地区):
US
年份:
2018
代理人:
摘要：
The present invention provides engineered phenylalanine ammonia lyase (PAL) polypeptides and compositions thereof, as well as polynucleotides encoding the engineered phenylalanine ammonia lyase (PAL) polypeptides. In some embodiments, the engineered PAL polypeptides are optimized to provide enhanced catalytic activity, as well as reduced sensitivity to proteolysis and increased tolerance to storage at elevated temperatures. In some embodiments, the engineered PAL polypeptides contain fewer phenylalanine residues than wild-type PAL polypeptides. The present invention also provides methods for the use of the compositions comprising the engineered PAL polypeptides for therapeutic and industrial purposes.
