A device for perfusing porous biomaterials with biological liquids includes a tubular body with an axial cavity defining a perfusion chamber for containing a porous biocompatible material, a connector at a distal end of the tubular body for receiving a perfusion liquid, a plunger having a distal end slidably movable in the cavity to withdraw air from the biomaterial, a vent for releasing the air withdrawn from the biomaterial and contained in the chamber, and a filter permitting selective passage of the air contained in the chamber toward the vent, while preventing leakage of the liquid to the outside. An air collector communicates with the chamber and the vent, and a control unit is associated with the plunger and designed to be actuated by the user to control the air flow from the air collector to the vent.A method of perfusing porous biomaterials with biological liquids.