A perfusion device and method is provided in various examples. In an example, the device includes a container having a first internal chamber configured to hold the material a port for introducing the liquid into the chamber a vent for releasing gas and liquid from the chamber and a flow control device for sealing the vent to allow a vacuum to be drawn on the first chamber. In an example, the container includes a syringe that defines the internal material chamber and includes an end cap and a plunger. The vent may be formed by a venting passageway in the plunger and/or the cap. In various examples, the vacuum may be created by a medical syringe coupled to the container. In some examples, the medical syringe may also be used to deliver the liquid into the container.
