Elena S. Revazova,Marina V. Pryzhkova,Leonid N. Kuzmichev,Jeffrey D. Janus
申请号：
US14945326
公开号：
US20160143956A1
申请日:
2015.11.18
申请国别(地区):
US
年份:
2016
代理人:
摘要：
Methods of producing human stem cells are disclosed for parthenogenetically activating human oocytes by manipulation of O2 tension, including manipulation of Ca2+ under high O2 tension and contacting oocytes with serine threonine kinase inhibitors under low O2 tension, isolating inner cell masses (ICMs) from the activated oocytes, and culturing the cells of the isolated ICMs under high O2 tension. Moreover, methods are described for the production of stems cells from activated oocytes in the absence of non-human animal products, including the use of human feeder cells/products for culturing ICM/stem cells. Stem cells produced by the disclosed methods are also described.
