The invention relates to a preparation method of conchiolin as well as water-soluble conchiolin and acid-soluble conchiolin prepared by virtue of the preparation method. The preparation method of the conchiolin comprises the following steps: 1) grinding pearls into pearl powder； 2) carrying out water bathing on the nanometer pearl powder by using deionized water, carrying out ultra-high pressure homogenization and low-temperature centrifugation to obtain a water-soluble pearl extract a and a precipitate, and washing the precipitate by using an ethanol solution for three times； 3) dissolving the obtained precipitate by using weak acid at a certain temperature, stirring at low speed to carry out reaction, and taking a supernatant to serve as an acid-soluble pearl extract b by virtue of low temperature centrifugation； 4) respectively desalting the water-soluble pearl extract a and the acid-soluble pearl extract b； and 5) after desalting, freeze-drying the water-soluble pearl extract a and the acid-soluble pearl extract b to respectively obtain the water-soluble conchiolin and the acid-soluble conchiolin. According to the preparation method disclosed by the invention, the nanometer pearl powder is utilized to provide a raw material for pearl skin care products and pearl health products.本發明涉及一種從珍珠中分離製備珍珠可溶食用鈣和珍珠複合美白因數溶液的方法，步驟如下：(1)將珍珠粉碎至奈米級，然後加入去離子水，通過壓力和溫度的協同，將珍珠粉進行微乳化；(2)然後將珍珠粉乳液進行電滲析，濃水池裝去離子水，淡水池裝珍珠粉乳液，進行迴圈脫鈣分離獲得含鈣鹽溶液和珍珠蛋白溶液；(3)鈣獲得含鈣鹽溶液作為可溶食用鈣或可溶食用鈣原料；(4)將獲得的珍珠蛋白溶液中加入磷酸鹽緩衝液，勻質；(5)首先預處理，然後將混合液置於反應鍋中，進行酶催化反應，反應結束後在沸水浴滅酶離心，去上清液，即為珍珠複合美白因數溶液。本發明方法的應用，極大的擴大珍珠粉的應用領域，並精細化珍珠粉的深加工應用。
