Jun Takahashi,Daisuke Doi,Bumpei Samata,Kiyotoshi Sekiguchi,Yuichi Ono
申请号：
US14916696
公开号：
US20160215260A1
申请日:
2014.09.04
申请国别(地区):
US
年份:
2016
代理人:
摘要：
The present invention provides a method for producing dopaminergic neuron progenitor cells from pluripotent stem cells, which method comprises the steps of: (i) performing adherent culture of pluripotent stem cells on an extracellular matrix in a medium containing a reagent(s) selected from the group consisting of BMP inhibitor, TGFβ inhibitor, SHH signal-stimulating agent, FGF8, and GSK3β inhibitor (ii) collecting Corin- and/or Lrtm1-positive cells from the cells obtained in Step (i) using a substance which binds to Corin and/or a substance which binds to Lrtm1 and (iii) performing suspension culture of the cells obtained in Step (ii) in a medium containing a neurotrophic factor.
