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NEW METHOD FOR INDUCING DOPAMINE-PRODUCING NEURAL PRECURSOR CELLS
专利权人:
EISAI R&D MANAGEMENT CO., LTD.;KYOTO UNIVERSITY
发明人:
TAKAHASHI, JUN,DOI, DAISUKE,SAMATA, BUMPEI,SEKIGUCHI, KIYOTOSHI,ONO, YUICHI
申请号:
SG11201601720R
公开号:
SG11201601720RA
申请日:
2014.09.04
申请国别(地区):
SG
年份:
2016
代理人:
摘要:
The present invention provides a method for producing dopaminergic neuron progenitor cells from pluripotent stem cells, which method comprises the steps of: (i) performing adherent culture of pluripotent stem cells on an extracellular matrix in a medium containing a reagent(s) selected from the group consisting of BMP inhibitor, TGF² inhibitor, SHH signal-stimulating agent, FGF8, and GSK3² inhibitor; (ii) collecting Corin- and/or Lrtm1-positive cells from the cells obtained in Step (i) using a substance which binds to Corin and/or a substance which binds to Lrtm1; and (iii) performing suspension culture of the cells obtained in Step (ii) in a medium containing a neurotrophic factor.
来源网站:
中国工程科技知识中心
来源网址:
http://www.ckcest.cn/home/

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