An apparatus for plant cultivation includes a sealed dark chamber (10) and a sealed lighted chamber (30) which accommodate a root portion and a stem and leaf portion of a plant (P) respectively The lighted chamber (30) is defined by a transparent housing above the dark chamber (10) and the lighting is provided by optical fibers. The dark chamber (10) is supplied with nourishment, oxygen and the like, while the lighted chamber (30) is supplied with CO2-containing air which is controlled to a predetermined temperature and humidity. The supply of light occurs on a scheduled basis in accordance with a state of stomata of the plant (P).