The present invention provides a method for preparing non-free radical photo-crosslinking hydrogel. The method comprises the following steps: dissolve a component A in a biocompatibility medium to obtain a solution A, and dissolve a component B such as a hydrazide, hydroxylamine or primary amine macromolecule derivative in the biocompatibility medium to obtain a solution B uniformly mix the solution A and the solution B to obtain a hydrogel precursor solution and when the hydrogel precursor solution is under illumination, an aldehyde group generated by o-nitrobenzyl in the component A in optical excitation and hydrazide, hydroxylamine or a primary amino group in the component B form hydrogel through cross-linking in a manner of respectively forming hydrazone, oxime, or a Schiff base. The present invention further provides a kit used for preparing hydrogel and applications of hydrogel in tissue repairing, beautifying, and serving as a cell, protein, or a drug carrier. According to the present invention, optical in situ gel can be implemented on a tissue surface, and especially, in situ thin glue can be implemented on a wound surface, so the present invention is particularly suitable for tissue repairing and isolation on a clinical wound surface.La présente invention concerne un procédé de préparation dhydrogel à photoréticulation non radicalaire. La présente invention se réfère en outre à une trousse de préparation dhydrogel et à des applications dhydrogel dans la réparation tissulaire, lembellissement et en tant que support de cellule, de protéine ou de médicament. Selon la présente invention, un gel optique in situ peut être formé sur une surface tissulaire, et notamment une fine couche de colle in situ peut être formée sur la surface dune plaie, la présente invention étant particulièrement appropriée à des fins de réparation tissulaire et disolement sur la surface dune plaie clinique.
