Recombinant phytases, protein preparation, heterodimer, immobilized polypeptide, arrangement, food supplement, edible enzyme release matrix and edible pellet comprising them, as well as methods of producing said enzymes and for hydrolyzing an inositol hexaphosphate, oil degreasing and grain processing
JAY M. SHORT,KEITH KRETZ,KEVIN A. GRAY,NELSON R. BARTON,JAMES B. GARRETT,EILEEN O'DONOGHUE,ERIC J. MATHUR
申请号：
BR0208851
公开号：
BRPI0208851B1
申请日:
2002.05.24
申请国别(地区):
BR
年份:
2015
代理人:
摘要：
A purified and modified phytase enzyme from Escherichia coli K12 appA phytase is provided. The enzyme has phytase activity and improved thermal tolerance as compared with the wild-type enzyme. In addition, the enzyme has improved protease stability at low pH. Glycosylation of the modified phytase provided a further improved enzyme having improved thermal tolerance and protease stability. The enzyme can be produced from native or recombinant host cells and can be used to aid in the digestion of phytate where desired. In particular, the phytase of the present invention can be used in foodstuffs to improve the feeding value of phytate rich ingredients.
