NICOLAS UGOLIN;YOANN FEDOR;IVANA JUKIC;VINCENT PAGET;SYLVIE CHEVILLARD;ARNAUD TUPINIER;EMILIE LEFEVRE
发明人:
NICOLAS UGOLIN,YOANN FEDOR,IVANA JUKIC,VINCENT PAGET,SYLVIE CHEVILLARD,ARNAUD TUPINIER,EMILIE LEFEVRE
申请号：
FR1402016
公开号：
FR3025605A1
申请日:
2014.09.08
申请国别(地区):
FR
年份:
2016
代理人:
摘要：
The loss and / or dilution of one or more antibodies of interest or part of an antibody, or defocusing, during the steps involving the antigen for: - Immunization of B lymphocyte stimulation, - Selection antibodies or antibody-producing cells； isolation of antibodies or antibody-producing cells； production of antibodies remains a limiting lock in antibody-producing technologies. To improve the focus of immunization and / or selection and / or isolation and / or production of one or more monoclonal or pseudo-monoclonal antibodies (different antibodies directed against the same epitope) or all parts of one or more monoclonal or pseudo-monoclonal antibodies recognizing an antigen of interest, we propose a polyvalent immunization and selection support and a new method using this support, for the production of monoclonal and pseudo-monoclonal antibodies. This polyvalent immunization and selection support comprises a diamond-fixing SP3 diamond carbon phase, so that the bond between the support and the antigen is via a covalent bond, preferably a carbon / carbon bond. carbon between a carbon of the diamond phase and a carbon of the antigen. The diamond support is only slightly immunogenic, the antigens being fixed by a carbon-carbon bond (non-peptide or otherwise), the immune response is focused on the antigen only. This support also improves antibody collection methods, in particular for phage display technologies. La perte et/ou la dilution d'un ou plusieurs anticorps d'intérêt ou partie d'un anticorps, ou défocalisation, durant les étapes faisant intervenir l'antigène pour : - l'immunisation de la stimulation des lymphocytes B, - la sélection des anticorps ou des cellules produisant les anticorps, - l'isolement des anticorps ou des cellules produisant les anticorps -la production des anticorps restent un verrou limitant dans les technologies de production d'anticorps. Pour améliorer la focalisation de l'immunisation et/ou de la sélection et/ou de l'iso
