The present invention provides an antibacterial essential oil comprising 30 to 70 parts by weight of an extract of Aquilaria agallocha Roxburgh, 30 to 70 parts by weight of camellia oil, 10 to 40 parts by weight of an extract of Dendropanax morbiferum Leveille, 10 to 40 parts by weight of an extract of Portulaca oleracea L., 10 to 40 parts by weight of an extract of Houttuynia cordata, 10 to 40 parts by weight of an extract of Salicornia herbacea, 10 to 40 parts by weight of an extract of glycyrrhizae radix, 10 to 40 parts by weight of a tea extract of Phytolacca americana L., 10 to 40 parts by weight of a jujube extract, 10 to 40 parts by weight of an extract of Paeonia japonica, and 10 to 40 parts by weight of an extract of Hinoki cypress based on 100 parts by weight of an extract of Juniperus chinensis. The antibacterial essential oil according to the present invention has effects of improving atopic dermatitis, acne and the like since a peculiar natural perfume of the antibacterial essential oil emitted by well combining respective merits of main ingredients including Juniperus chinensis, aloeswood, camellia oil, Dendropanax morbiferum Leveille, Portulaca oleracea L., Houttuynia cordata, Saururuschinensis, Salicornia herbacea, glycyrrhizae radix, Phytolacca americana L., jujube, Paeonia japonica, Hinoki cypress, and others is comfortable like scent of wild chrysanthemum, and the antibacterial essential oil not only emits a soft fragrance of fields and mountains, but also is harmless to the human body and provides antibacterial properties.COPYRIGHT KIPO 2017본 발명은 향나무 추출물 100중량부 기준으로, 침향나무 추출물 30 내지 70중량부; 동백기름 30 내지 70중량부; 황칠나무 추출물 10 내지 40중량부; 마치현 추출물 10 내지 40중량부; 어성초 추출물 10 내지 40중량부; 함초 추출물 10 내지 40중량부; 감초 추출물 10 내지 40중량부; 우방차 추출물 10 내지 40중량부; 대추 추출물 10 내지 40중량부; 백작약 추출물 10 내지 40중량부; 및 편백나무 추출물 10 내지 40중량부를 포함하는 항균성 에센셜 오일을 제공한다.본 발명에 따른 항균성 에센셜 오일은 향나무, 침향, 동백기름, 황칠나무, 마치현, 어성초, 삼백초, 함초, 감초,우 방차, 대추, 백작약, 편백나무 등 주원료의 각 장점이 잘 융합되어 발산하는 특유의 천연향이 들국화 향처럼 편안하고,