The utility model provides two blood glucose test structures that can use what biological sensor,Including an insulating substrate; Electrode (working electrode and reference electrode) on insulating substrate,The both ends of the electrode are respectively ferment reactive end and biological sensor connecting pin; One padded of insulation,Top have one it is U-shaped within concane gap and insulating substrate form ferment reaction zone,At least one layer of ferment reaction film is coated in the ferment reaction zone; One upper cover plate,The ferment reaction chamber in a three-dimensional space is collectively formed with padded of insulation and insulating substrate; It is sample suction inlet that the top of the upper cover plate, which has half moonpool,; The upper cover plate corresponds to what and insulate the bottom three-dimensional space of padded U-shaped interior concane gap,It is the exhaust outlet of sample sucking with the small opening of a rectangle,Also the form of ferment reaction chamber whether can be full of for sample.本新型提供二例可用於生物感測器之血糖試片結構,包括一絕緣基板;絕緣基板上之電極(工作電極及參考電極),該電極之兩端分別為酵素反應端以及生物感測器連接端;一絕緣墊高片,頂端具有一U型之內凹缺口與絕緣基板形成酵素反應區,該酵素反應區內塗佈有至少一層酵素反應膜;一上蓋片,與絕緣墊高片及絕緣基板共同形成一個三度空間之酵素反應室;該上蓋片之頂端具有一半月形開口為樣品吸入口;該上蓋片對應於絕緣墊高片U型內凹缺口之底部三度空間,具有一長方形小開口為樣品吸入之排氣口,亦可為樣品是否充滿酵素反應室之視窗。