THE U.S.A.; AS REPRESENTED BY THE SECRETARY OF THE ARMY; ON BEHALF OF WALTER REED ARMY INSTITUTE OFRESEARCH
发明人:
ZOLLINGER, WENDELL DAVID,DONETS, MIKHAIL,SCHMIEL, DEBORAH,IONIN, BORIS,MARQUES, RYAN,MORAN, ELIZABETH ELLEN
申请号：
NZ58981209
公开号：
NZ589812A
申请日:
2009.06.01
申请国别(地区):
NZ
年份:
2012
代理人:
摘要：
Disclosed is a vaccine comprising native outer membrane vesicles (NOMVs) obtained from at least two meningococcal strains that have been genetically modified to provide broad based protection, wherein the native outer membrane vesicles include different sets of antigens, wherein the different sets of antigens comprise one or more PorA proteins, one or more LOS, and one or more conserved outer membrane proteins wherein the genetically modified strains have been modified to provide enhanced safety based on inactivation of lpxL1, synX, and IgtA genes and wherein at least one of the genetically modified strains expresses at least two different PorA subtype proteins or subtype epitopes. Further disclosed is a genetically modified vaccine strain of Neisseria meningitidis subtype B comprising the genetic modifications of: i) inactivation of a synX gene, ii) inactivation of the lpxL1 gene, iii) inactivation of the lgetA gene, iv) insertion of a second porA gene in the place of a opaD gene, v) increased expression of NadA compared with the native strain or increased expression of factor H binding protein variant 1 or 2 and vi) stabilized increased expression of Opc and PorA proteins.
