A specimen collection container (10) having a transitional fill volume indicator (30) is disclosed. The specimen collection container includes an open top end (12) a closed bottom end (14) and a sidewall (16) extending therebetween defining an interior (18). The specimen collection container includes a transitional fill volume indicator adjacent the sidewall such that the container defines a first volumetric interior (32) defined by the sidewall between the closed bottom end and the transitional indicator. The specimen collection container also defines a second volumetric interior (34) defined by the sidewall between the closed bottom end and a portion of the sidewall disposed above the transitional indicator. The first volumetric interior is adapted to allow extraction of a specimen therefrom by a first extraction process and the second volumetric interior is adapted to allow extraction of a specimen therefrom by either the first extraction process or a second extraction process the second extraction process being different than the first extraction process.