Disclosed are a perfusion culture method and a perfusion culture device whereby a perfusate can be delivered to all parts of an organ or tissue so that the organ or tissue can be preserved while sufficiently maintaining the function thereof. Specifically disclosed is a method for perfusion culture of an organ or tissue removed from a mammal, said organ or tissue having been removed together with a second organ or tissue connected to the aforesaid organ or tissue in vivo, which comprises a step for fixing said second organ or tissue so as to hang the aforesaid organ or tissue and a step for perfusing blood vessels in the aforesaid organ or tissue with a perfusate.本發明的技術課題係在於提供:藉由將灌流液行遍臟器或組織的各角落,而在於可充分維持其功能的狀態下,能夠長時間保存臟器或組織之灌流培養方法以及灌流培養裝置。本發明之用來解決技術課題之手段的灌流培養方法,係將從哺乳類動物摘取出來的臟器或組織予以灌流培養的方法,前述臟器或組織係和在活體內與前述臟器或組織相連的第二臟器或組織一起被摘取出來的,包含:藉由將前述第二臟器或組織加以固定,而將前述臟器或組織予以吊掛的工序、以及將灌流液灌流到前述臟器或組織的血管的工序。
