A method of culturing Antrodia cinnamomea and obtaining Antrodia cinnamomea with improved contents of antcin C and zhankuic acid C is disclosed in the invention. The method comprises: providing a wood segment of Cinnamomum kanehirai inoculating a strain of Antrodia cinnamomea to the wood segment, to obtain the wood segment inoculated with the strain of Antrodia cinnamomea with 0.162 g dry weight of hyphae and mycelia of the strain of Antrodia cinnamomea per kg wood segment and irradiating the wood segment inoculated with the strain of Antrodia cinnamomea by a light of wavelength of 600-700 nm to obtain a fruit body wherein an irradiation time of the wood segment inoculated with the strain of Antrodia cinnamomea is 8-12 hours per day for 30-40 days.
