DR PRANAYWAL,DR GAURAV TIWARI,DR RUCHI TIWARI,ANKITAWAL,CHITRANSHU GUPTA
申请号：
IN201611033236
公开号：
IN201611033236A
申请日:
2016.09.29
申请国别(地区):
IN
年份:
2018
代理人:
摘要：
The objective of the research work was to design novel liposomes containing both hydrophilic and hydrophobic drug by using modified ethanol ejection method was used to formulate liposomes. The liposome formulation F6 showed higher drug entrapment and controlled drug release. Small multilamellar vesicles, with sizes ranging from about 200 to 400 nm, were successfully obtained. Results indicated a significant influence of phospholipid amounts on liposome size and encapsulation efficiency. Due to their composition variability and structural properties, liposomes are extremely versatile leading to a large number of applications including pharmaceutical, cosmetics and food industrial fields. Liposome morphology was studied by SEM, liposomes ranged in size from 200 to 400 nm. No drug crystals were visible in SEM-images, regardless of the preparation technique or the loaded drug. FT-IR graphs of the pure drug, soya lecithin, cholesterol and their physical mixtures was performed and the FT-IR graphs are shown in from the figures it is noted that there is no possible interactions between drugs and the other. The average percent drug entrapment efficiency of F6 showed a maximum drug entrapment of 72.86% and 69.70% for 5FU and TTN respectively. The drug release profile of F6 showed a best fit to the desired control drug release profile among all the formulations. Size and encapsulation efficiency Phospholipid concentration showed a positive effect and it was found that when phospholipid concentration was increased from 20 to 60 mg/ml, the encapsulation efficiencies of formulation increased. A study showed that liposomes were not stable at high temperature but formulations are most stable when stored at lower temperature i.e. 4oC. So, in the liposomes we were successfully incorporated both hydrophilic and hydrophobic drugs and it can be further used for formulation development.
