THE UNITED STATES OF AMERICA AS REPRESENTED BY THE SECRETARY OF THE NAVY
发明人:
BOSS PAMELA A.,SORENSEN KARA C.,GEORGE ROBERT D.,OBRAZTSOVA ANNA Y.,OILER JONATHON K.
申请号：
US201615274599
公开号：
US2018088113(A1)
申请日:
2016.09.23
申请国别(地区):
美国
年份:
2018
代理人:
摘要：
A method uses flow cytometry to prepare surface enhanced Raman scattering (SERS) substrates for obtaining SERS spectra of bacteria. The method involves using a flow cytometer to sort bacterial cells into populations of bacterial cells based upon their biophysical characteristics. The cells may then be washed with a borate buffer to remove any chemical species that degrade the SERS response. A colloid-coated bacteria suspension is then created by mixing one of the populations of bacterial cells with SERS-active colloidal particles. The colloid-coated bacteria suspension is incubated until the SERS-active colloidal particles partition through the capsule and bind to the cell wall for each bacterial cell in the colloid-coated bacteria suspension. The colloid-coated bacteria suspension is then disposed onto a filter and a SERS spectra of the colloid-coated bacteria suspension is obtained using a Raman spectrometer.
