PURPOSE: A method for preparing policosanol ester and an additive containing polycosanol ester are provided to prevent crystallization of policosanol ester and to ensure various functionalities. CONSTITUTION: A method for preparing policosanol ester comprises: a step of mixing policosanol with 20-40 carbon atoms and unsaturated fatty acid; and a step of reacting the mixture with lipase at 50-90 deg. C Policosanol ester is Candida antarctica-derived lipase, Rhizomucor miehei-derived lipase, or Thermomyces lanuginosus-derived lipase. Policosanol is tetracosanol, pentacosanol, hexacosanol, heptacosanol, octacostanol, nonacosano, triacontanol, dotriacontanol, tetratriacontanol, hexatriacontanol, or octatriacontanol. The unsaturated fatty acid is oleic acid, conjugated linoleic acid, vaccenic acid, linoleic acid, pinolenic acid, eicosenoic acid, docosatetraenoic acid, or docosahexaenoic acid. [Reference numerals] (AA) Concentration of polycosanol ester(weight%); (B1) Embodiment 1; (B2) Embodiment 2; (B3) Embodiment 3; (C1) Comparative embodiment 1; (C2) Comparative embodiment 2; (C3) Comparative embodiment 3; (C4) Comparative embodiment 4; (DD) Enzyme본 발명은 폴리코사놀 에스터, 이의 제조 방법 및 이를 포함하는 첨가제에 관한 것으로, 구체적으로 폴리코사놀 및 불포화 지방산이 에스터 결합된 폴리코산올 에스터, 폴리코사놀 및 불포화 지방산을 리파아제와 반응시키는 폴리코사놀 에스터의 제조 방법 및 폴리코사놀 에스터를 포함하는 첨가제에 관한 것이다.