Provided herein are conditionally activated polypeptide constructs comprising a protease-activated domain binding to CD3, at least one half-life extension domain, and two or more domains binding to one or more target antigens. Also provided are pharmaceutical compositions thereof, as well as nucleic acids, recombinant expression vectors and host cells for making such polypeptide constructs. Also disclosed are methods of using the disclosed polypeptide constructs in the prevention, and/or treatment diseases, conditions and disorders.本發明提供經條件性活化之多肽構建體，該多肽構建體包含與CD3結合之經蛋白酶活化的結構域、至少一個半衰期延長結構域及二或更多個與一或多個靶抗原結合之結構域。亦提供該多肽構建體之醫藥組成物及用於製備該多肽構建體之核酸、重組表現載體和宿主細胞。亦揭示使用該揭示之多肽構建體以預防及/或治療疾病、病症和失調之方法。
