RAO, GALLA CHANDRA,雷 盖拉,雷 蓋拉,CONNELLY, MARK CARLE,康娜莉 马克,康娜莉 馬克
申请号：
TW100137078
公开号：
TWI577389B
申请日:
2011.10.13
申请国别(地区):
TW
年份:
2017
代理人:
摘要：
A highly sensitive assay is disclosed which combines immunomagnetic enrichment with multiparameter flow cytometric or image cytometry to detect, enumerate and characterize carcinoma cells in the blood. The present invention incorporates the conjugation of different antibodies to the same ferrofluid. This has the effect of making the ferrofluid polyspecific with respect to the antigens that the ferrofluid will bind. The multiple antibodies present on the same ferrofluid do not appear to block or otherwise interfere with each other. Such ferrofluids have the highly desirable effect of being able to bind specifically to more than one type of cell. The assay is especially useful to enable the capture of CTCs that have low EpCAM expression, but high expression of other tumor markers accordingly, the assay facilitates the biological characterization and staging of carcinoma cells.本發明揭示一高靈敏度的測定,該測定將具多參數流動式細胞儀或影像細胞分析術的免疫磁性富集合併以檢測、計數以及特性化於血液中的惡性腫瘤細胞。本發明把不同抗體的結合作用引入至相同的鐵磁流體。根據鐵磁流體欲結合的抗原,此具有使鐵磁流體產生多專一性的效果。呈現在相同的鐵磁流體上的多重抗體並非看來像是阻塞,或者相互干擾。此等鐵磁流體具有能夠特定地結合一個以上細胞類型之非常想要的效果。測定尤其是對捕捉CTCs很有用處,該等CTCs具有低EpCAM表現,但對其他腫瘤標記則具有高表現。因此,測定有助於使惡性腫瘤細胞的生物學特性化並分期。
