The present invention provides a kind of aqueous liquid pharmaceutical formulations, it includes people's anti-TNF alpha antibodies or its antigen-binding portion thereofs, the dosage form mitigates pain at least about 50% relevant to injection in subject when compared with injecting the preparation identical in other respects comprising at least one salt and/or at least one buffer. The present invention also provides a kind of aqueous liquid pharmaceutical formulations, and it includes people's anti-TNF alpha antibodies or its antigen-binding portion thereofs, and into object, Hou has the bioavilability improved in subcutaneous administration. The preparation may include therapeutic protein, such as people's anti-TNF-α antibody or its antigen-binding portion thereof or its biological imitation medicine.本發明提供了一種水性的液體藥物製劑，其包含人抗TNFα抗體或其抗原結合部分，所述劑型當與注射包含至少一種鹽和／或至少一種緩衝劑的在其他方面相同的製劑相比時，减輕受試者中與注射相關的疼痛至少約50％。本發明還提供了一種水性的液體藥物製劑，其包含人抗TNFα抗體或其抗原結合部分，並在皮下施用到對象中後具有提高的生物利用度。所述製劑可以包含治療性蛋白質，例如人抗TNF-α抗體或其抗原結合部分或者其生物仿製藥。
