FONDAZIONE ISTITUTO INSUBRICO DI RICERCA PER LA VITA
发明人:
ANNA TARAVELLA,LINDA CAVALETTI,LUCIA CARRANO,MARA BRUNATI,MONICA ABBONDI
申请号:
BR112014012100
公开号:
BR112014012100A2
申请日:
2012.11.05
申请国别(地区):
BR
年份:
2017
代理人:
摘要:
Abstract: "Proteases capable of hydrolyzing acidic gluten peptides and proteins of actinomycete actinoallomurus". The present invention relates to a novel family of proteolytic enzymes having the ability of hydrolysis at a pH of between 3 and 8 gluten oligopeptides that are resistant to cleavage by gastric and pancreatic enzymes and whose presence in the intestinal lumen results in toxic effects. The enzymes have been identified as the s8 / s53 endopeptidases and are produced by an actinoallomurus strain. The object of the invention also includes methods for producing the enzyme composition comprising endopeptidases by culturing native, mutant actinoallomurus strains, or recombinant host cells comprising encoding nucleic acids for endopeptidases. said nucleic acids constitute a further object of the invention. enzyme compositions comprising at least one endopeptidase of the invention which are useful for the treatment and / or prevention of celiac psyllosis, dermatitis herpetiformis and any other gluten intolerance disorder as ingredients of pharmaceutical formulations or as food additives and drinks.resumo patente de invenção: "proteases capazes de hidrolisar peptídeos e proteínas de glúten em ph ácido, do actinomiceto actinoallomurus". a presente invenção refere-se a uma nova família de enzimas proteolíticas possuindo a capacidade da hidrólise em um ph entre 3 e 8 oligopeptídeos do glúten que são resistentes à clivagem por enzimas gástricas e pancreáticas e cuja presença no lúmen intestinal resulta em efeitos tóxicos. as enzimas foram identificadas como as endopeptidases da família s8/s53 e são produzidas por uma cepa de actinoallomurus. o objetivo da invenção inclui também métodos para produzir a composição de enzimas compreendendo as endopeptidases pelo cultivo de cepas de actinoallomurus nativas, mutantes das mesmas, ou células hospedeiras recombinantes compreendendo a codificação de ácidos nucleicos para as endopeptidases. os ditos ácidos nucleicos constituem