This invention provides a positioning mark device for surgery navigation system and applying instrument thereof. The positioning mark device comprises a positioning base and a frequency modulation type radio frequency positioning module. One end of the positioning base is a locking portion having a screw structure and the other end is a head portion having an assembly structure. The free end of the head portion has an engagement structure. The frequency modulation type radio frequency positioning module has a sleeve that is fitted to the structure of the head portion formed an buckling engagement with the assembly structure. The frequency modulation type radio frequency positioning module comprises an antenna unit, a printed circuit board unit electrically connected with the antenna unit, a battery unit for supplying power to the electric components of the printed circuit board unit. The frequency modulation type radio frequency positioning module can send back a radio frequency feedback signal outwardly according to a received frequency modulation frequency signal.一種手術導航系統之定位標記裝置及其實施器械，定位標記裝置包含定位基座及調頻式射頻定位模組，定位基座一端為一具有螺絲結構的鎖部，另一端為一具有組接結構的頭部，頭部之自由端具有一嵌合結構；調頻式射頻定位模組具有一配合頭部結構並與組接結構形成卡扣的套接，調頻式射頻定位模組包含一天線單元、一電性連接天線單元的印刷電路板單元、一供電予印刷電路板單元的電子元件電池單元，該調頻式射頻定位模組係可根據所接收之一調頻頻率訊號，對外回傳包含一無線射頻的反饋信號。
