This invention relates to bacterial cellulose-based ice bags and their production methods thereof. Bacterial cellulose membranes used in the present invention are produced through the fermentation of cellulose-producing microorganisms. Ice bags with three sealed sides and one open side are made from water-impermeable (under room temperature and normal atmospheric pressure) bacterial cellulose membranes. The bags are filled with filler materials and then sterilized at 120 degrees C. for 30 minutes and sealed aseptically. The use of bacterial cellulose as the packaging material solves the problems associated with conventional ice bags. The advantages of the present invention include: no condensation at the bag surface, smaller temperature changes during the course of application, and longer lasting ice therapy session. In addition, bacterial cellulose membrane is safe and non-toxic, and has excellent biocompatibility, plasticity and higher mechanical strength, making it an ideal packaging material for ice bags.