A circuit board secure connection apparatus, a circuit board and a plug-and-lock industrial computer frame are provided. The circuit board secure connection apparatus includes: a holder; a connector which is elastic and movably mounted on the holder; a profile ratchet which is elastic and hinged on a bottom portion of the holder through a first hinge; a hook handle hinged on a rear portion of the holder through a second hinge; and a hook button which is elastic and movably mounted on the hook handle; wherein the hook handle cooperates with the holder through the hook button for being attached to or detached from the holder; the hood handle cooperates with the profile ratchet for rotating the profile ratchet. The plug-and-lock industrial computer frame with the circuit board secure connection apparatus and the circuit board has advantages such as simple installation, reliable connection, and sufficient performance.
