Methods for evaluating malignant cells in an epidermis in vitro, and for evaluating test compounds for human toxicity.

• 收藏
• 分享

专利权人:

WISCONSIN ALUMNI RESEARCH FOUNDATION

发明人:

LYNN ALLEN-HOFFMANN,SANDY J. SCHLOSSER,MICHAEL A. PICKART

申请号：

BR9912047

公开号：

BR9912047B1

申请日:

1999.07.12

申请国别(地区):

BR

年份:

2014

代理人:

摘要：

A spontaneously immortalized human keratinocyte cell line is disclosed. In a preferred embodiment, this cell line is ATCC 12191. In another embodiment of the invention, a method of assaying the effect of a test tumor cell modulation agent is disclosed. The method comprises the steps of obtaining a human stratified squamous epithelial cell culture, wherein the culture comprises human malignant squamous epithelial cells and spontaneously immortalized human keratinocytes, wherein the culture forms a reconstituted epidermis. One then treats the epidermis with a test tumor cell modulation agent and evaluates the growth of the malignant cells within the epidermis.

来源网站：

中国工程科技知识中心

来源网址：

http://www.ckcest.cn/home/