A needle positioning means includes a flexible slice body and a plurality of guiding holes formed on the flexible slice body. The flexible slice body has two opposite surfaces and an edge, wherein the plurality of guiding holes has openings on the surfaces. At least one diameter of the guiding hole is greater than or equal to an outer diameter of the needle. The plurality of guiding holes are spaced at intervals and evenly distributed on the flexible slice body.一種針頭定位裝置,包含可撓性片體以及複數個導孔形成於該可撓性片體。可撓性片體具有分別於相反側之兩表面以及一側緣,複數個導孔係開口於該兩表面。該些導孔之至少一孔徑大於等於針頭之外徑,且係相互間隔並均勻分布於可撓性片體上。1a‧‧‧針頭定位裝置10‧‧‧可撓性片體11‧‧‧表面12‧‧‧側緣20‧‧‧導孔21‧‧‧開口