An apparatus for positioning a puncture needle is described, which is suitable to adjust and position the puncture needle. The apparatus for positioning the puncture needle includes a back frame and a positioning mechanism. The positioning mechanism is disposed on the back frame. The positioning mechanism includes a position-adjusting structure and an angle-adjusting structure. The position-adjusting structure can move relative to the back frame along a first direction and a second direction. The position-adjusting structure includes a first threading plate. The angle-adjusting structure is disposed on the position-adjusting structure and can move relative to the position-adjusting structure. The angle-adjusting structure includes a second threading plate. The puncture needle can be put in the second threading plate and the first threading plate.一種穿刺針定位裝置。此穿刺針定位裝置適用以調整並定位穿刺針。穿刺針定位裝置包含背架以及定位機構。定位機構係設置在背架上。定位機構包含位置調整結構以及角度調整結構。位置調整結構可沿著第一方向與第二方向相對背架移動。位置調整結構包含第一穿針片。角度調整結構設置在位置調整結構上,並可相對位置調整結構移動。角度調整結構包含第二穿針片。穿刺針可穿設於第二穿針片與第一穿針片中。100‧‧‧穿刺針定位裝置200‧‧‧穿刺針300‧‧‧背架400‧‧‧定位機構420‧‧‧位置調整結構421‧‧‧第一滑軌422‧‧‧第一滑塊423‧‧‧第二滑軌424‧‧‧第二滑塊424a‧‧‧第一凹槽424b‧‧‧第二凹槽425‧‧‧定位塊425a‧‧‧定位部426‧‧‧第一穿針片440‧‧‧角度調整結構441‧‧‧第三滑軌442‧‧‧第三滑塊443‧‧‧第四滑軌444‧‧‧第四滑塊445‧‧‧第二穿針片D1‧‧‧第一方向D2‧‧‧第二方向H‧‧‧距離