A double-sided needle groove, a frame body and a puncture frame, wherein the double-sided needle groove has an axisymmetric outer contour, and is provided at one side with at least one needle slot a (1) and at the other side with at least one needle slot b (2) and the needle slot b (2) is fan-shaped. The double-sided needle groove is provided with one or more needle slots a (1) having a fixed needle insertion angle and a needle slot b (2) having an adjustable needle insertion angle, and the needle slot a (1) and the needle slot b (2) respectively correspond to the diameters of puncture needles and may be designed to meet multiple width specifications. During the performance of a B-ultrasound puncture operation, based on operation requirements of a surgeon for a fixed or adjustable angle, there is only a need to switch front and back sides of the double-sided needle groove, thus avoiding the need for an additional puncture frame, and switch the needle slots according to the diameters of the puncture needles. As can be seen, the double-sided needle groove can be applied to various types of B-ultrasound puncture operations and can effectively reduce the number of accessories in the puncture operations, thereby providing fast installation, saving resources and reducing costs.
