1. A method for identifying one or more amino acid residues for substitution in a particular position in immunobindere which comprises: a) providing a first database of amino acid sequences vulgaris / or V, grouped according to subtype b) providing a second database of amino acid sequences vulgaris / or V, grouped according to subtype and selected as having at least one desirable functional property wherein the desired functional property is improved stability, solubility, decrease aggregatable improve expression and / or improve refolding c) determining amino acid frequency of occurrence of amino acid residues in the framework position of the first database and a corresponding framework position of the second database d) determining the enrichment factor (RF2: RF1) amino acids by building a relationship between the relative frequency of a residue in the second database (RF2) and the relative frequency of the balance at first base but nnyh (RF1) e) identifying amino acid residue (i) as a preferred amino acid residue for substitution at corresponding amino acid position immunobindera if the amino acid has the enrichment factor is greater than 1, or (ii) as an amino acid residue which must be removed from the corresponding amino acid position immunobindera if the amino acid residue had enrichment factor of less than 1.2. A method according to claim 1, wherein the amino acid residue of step d) has an enrichment factor of 4 to 6.3. A method according to claim 1 or 2, wherein the first database includes embryonic amino acid sequences V, Vili vulgaris V.4. A method according to claim 11. Способ идентификации одного или более аминокислотных остатков для замены в определенном положении в иммунобиндере, который включает:a) обеспечение первой базы данных аминокислотных последовательностей VH и/или VL, сгруппированных в соответствии с подтипомb) обеспечение второй базы данных аминокислотных последовательностей VH и/или VL, сгруппированных в соответствии с подтипом и выбранных,
