The present application provides methods of obtaining a plurality of T cell receptors specifically recognizing a target tumor antigen peptide from an individual that has clinically benefitted from an immunotherapy, such as Multiple Antigen Specific Cell Therapy. Tumor-specific TCRs, engineered immune cells expressing the TCRs and methods of treating a disease using the engineered immune cells are also provided.本申請案提供獲得複數種特異性辨識來自個體之目標腫瘤抗原肽之T細胞受體的方法，該個體已在臨床上受益於免疫療法，諸如多抗原特異性細胞療法。亦提供腫瘤特異性TCR、表現該等TCR之經工程改造之免疫細胞、及使用該等經工程改造之免疫細胞治療疾病的方法。
