The present application provides methods of preparing a population activated T cells by co-culturing T cells with dendritic cells loaded with a plurality of tumor antigen peptides. Also provided are methods of treating cancer in an individual using the activated T cells, pharmaceutical compositions and kits for cell-based cancer immunotherapy.本申請案提供藉由將T細胞與載有複數種腫瘤抗原肽之樹突細胞共培養來製備活化T細胞群之方法。亦提供的是使用該等活化T細胞治療個體中癌症之方法、醫藥組成物、及套組以用於基於細胞的癌症免疫療法。