The present application provides methods of preparing tumor antigen-specific T cells comprising enriching activated T cells from a first co-culture comprising a first population of antigen-loaded dendritic cells loaded and a population of T cells, and co-culturing the enriched activated T cells with a second population of antigen-loaded dendritic cells. Also provided are methods of treating cancer in an individual using the tumor antigen-specific T cells, pharmaceutical compositions and kits for cell-based cancer immunotherapy.本申請案提供製備腫瘤抗原特異性T細胞之方法,該方法包含:富集來自第一共培養物之活化T細胞,該第一共培養物包含第一抗原負載樹突細胞群負載及T細胞群;及將該等經富集之活化T細胞與第二抗原負載樹突細胞群共培養。亦提供的是使用該等腫瘤抗原特異性T細胞治療個體中癌症之方法、醫藥組成物、及套組以用於基於細胞的癌症免疫療法。