ris Interleukin 6receptor/interleukin 6 protein and its biologically active analogues their method of manufacture DNA and organisation DNA vector transformed mammalian cells and pharmaceutical composite
MICHEL REVEL,JUDITH CHEBATH,TSVEE LAPIDOT,ORIT KOLLET
申请号：
EEP200000012
公开号：
EE05519B1
申请日:
1998.07.09
申请国别(地区):
EE
年份:
2012
代理人:
摘要：
A chimeric glycosylated soluble interleukin-6 receptor (sIL-6R)-interleukin-6 (IL-6) protein (sIL-6R/IL-6) and biologically active analogues are claimed which comprise a fusion protein product between all of the naturally occurring form of sIL-6R and all of the naturally occurring form of IL-6, the sIL-6/IL-6 and analogues being glycosylated in a similar fashion to the glycosylation of naturally occurring sIL-6R and IL-6. Also claimed are: (1) a DNA sequence encoding a chimeric sIL-6R/IL-6 protein and biologically active analogues as above； (2) a DNA vector comprising a DNA sequence encoding a chimeric sIL-6R/IL-6 protein and biologically active analogues as above, the vector being suitable for expression of the chimeric protein in mammalian cells； (3) transformed mammalian cells containing a DNA vector as in (2) which are capable of expressing the sIL-6R/IL-6 chimeric protein sequence carried by the vector and of fully processing the expressed protein and secreting it into the culture medium in which the cells are grown.
