Brennan Miles B.,Spiegel Erin K.,Staerz Uwe D.,Wall Charles,Wheeler Janae,Maslanik William J.,Zhang Xianghua
申请号：
US201514921421
公开号：
US9719107(B2)
申请日:
2015.10.23
申请国别(地区):
美国
年份:
2017
代理人:
Greenberg Traurig, LLP `Pham Chinh H.`Xie Fang
摘要：
The embodiments disclosed herein relate to the construction of fully-deleted Adenovirus-based gene delivery vectors packaged without helper Adenovirus, and more particularly to their use in gene therapy for gene and protein expression, vaccine development, and immunosuppressive therapy for allogeneic transplantation. In an embodiment, a method for propagating an adenoviral vector includes (a) providing an Adenovirus packaging cell line; (b) transfecting a fully-deleted Adenoviral vector construct into the cell line; and optionally (c) transfecting a packaging construct into the cell line, wherein the fully-deleted Adenoviral vector construct and optionally the packaging construct can transfect the Adenovirus packaging cell line resulting in the encapsidation of a fully-deleted Adenoviral vector independent of helper Adenovirus. In an embodiment, a target cell is transduced with the encapsidated fully-deleted Adenoviral vector for treating a condition, disease or a disorder.
