There is provided a cannula holder capable of securing a cannula to the holder at the time of piercing work, and easily removing the cannula from the holder using tweezers or the like. The cannula holder of the present invention to which a piercing needle (30) for piercing an eyeball and a cannula (20) are attached when attaching the cannula used in ophthalmic operations to the eyeball includes claw portions (11), which are formed on a front end of the cannula holder (10) and press sides of the cannula (20) either at positions where said claw portions face each other or from multiple directions. A depression (12) may be provided in a side of the front end of the cannula holder (10), allowing visibility of a part of a back end of the cannula (20) from the depression (12).