There are provided: a preservative composition and a preservation liquid which can improve the shelf life of fruit and vegetables and which are safer to the human body; and fruit and vegetables treated with the preservation liquid. The present invention relates to a preservative composition for fruit and vegetables, the preservative composition containing an organic acid and/or a salt thereof, and at least one selected from the group consisting of glucosamine, glucosamine derivatives, chitin oligosaccharides, chitosan oligosaccharides, and salts thereof. Moreover, the present invention relates to a preservation liquid for fruit and vegetables, the preservation liquid containing the preservative composition and a liquid medium. Further, the present invention relates to fruit and vegetables treated with the preservation liquid.