GESELLSCHAFT FUER BIOTECHNOLOGISCHE FORSCHUNG MBH.
发明人:
GERTH KLAUS,HOEFLE GERHARD,REICHENBACH HANS,STEINMETZ HEINRICH
申请号：
HU0000497
公开号：
HU229833B1
申请日:
1997.11.18
申请国别(地区):
HU
年份:
2014
代理人:
摘要：
Epothilone derivatives are obtained by: (1) cultivation of Sorangium cellulosum DSM 6773 in the presence of an absorption resin； (2) separation of the resin and culture followed by washing with aqueous methanol； (3) eluting the washed resin with methanol and reducing the extract； (4) partitioning the extract between methanol and hexane； (5) reducing the methanolic phase and fractionating it on a Sephadex column, and (6) isolation of the epothilones by chromatography using a methanol/water mixture. Epothilone A is followed by epothilone B, and two further fractions.
