The present invention provides a fermented black soybean composition and manufacture method thereof. The fermented black soybean composition is produced by fermenting black soybean medium with gram positive bacillus. The fermented black soybean composition has the effect of triggering apoptosis in cancer cells so that it has the use for the preparation of a medicament for the treatment of cancer.本發明係提供一種黑豆發酵組合物及其製造方法,其中該黑豆發酵組合物係透過以革蘭氏陽性桿菌發酵黑豆液而製成。本發明之黑豆發酵組合物有誘發癌症細胞凋亡的能力,因而具有作為製備治療癌症之藥品的用途。(無元件符號說明)